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Home / Proteins & Peptide

KRAS (G12D), Isoform A, His-Tag, GDP-Loaded

SKU : BHP18201064

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BPS Bioscience

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Mfr.No.	101312
Description	Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end). In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease Biohippo #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column. Constructs: KRAS (G12D) (His-TEV-2-186)-(GDP)
Immunogen Region	2-186
Tag	N-terminal His-tag
Formulation	20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed according to the KRAS (G12C) Coupled Nucleotide Exchange Assay Kit (Biohippo #78004) with GDP loaded KRAS (G12C) (Biohippo #100640) and GDP loaded KRAS (G12D) (Biohippo #101312) titrated from 200 nM to 0 nM. Enzyme was incubated with SOS1; positive results show that SOS1 mediated exchange and subsequent RAS-Raf binding is successful. Avoid freeze/thaw cycles.
GenBank	NM_033360
Mw(kda)	23 kDa

Synonyms	C-K-RAS, CFC2, K-ras, kRas(G12D), kRas, KRAS proto-oncogene, GTPase, Kirsten Rat Sarcoma virus, NS, NS3, OES, RALD, KRAS1, KRAS2, RASK2, KI-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, c-Ki-ras, c-Ki-ras2
Uniprot	P01116

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201064	101312	4 x 50 ug	$1182.50		Add to Cart

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