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Home / Proteins & Peptide

KMO, His-FLAG-Tags

SKU : BHP18200009

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Mfr.No.	11307
Description	Human KMO, also known as Kynurenine 3-Monooxygenase, Kynurenine 3-Hydroxylase, and EC 1.14.13.9, Genbank Accession No. NM_003679, a.a. 1-486 (end) with C-terminal His-FLAG-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 58 kDa. host species: insect Host species: insect Host cell line: Sf9 cells Specific activity: 100 pmol/min/ug
Immunogen Region	1-486 (end)
Tag	C-terminal His-FLAG-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 100 ug/ml FLAG peptide, 0.2 mM TCEP and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	KMO #11307 was tested with various amounts of enzyme. The reactions in KMO Assay Buffer (50 mM Sodium Phosphate, pH 7.5, 0.1% (w/v) Brij-35) contains 200 uM NADPH, 400 uM L-Kynurenine, and various concentrations of KMO. The reaction mixture incubated for 90 minutes while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals at 340 nm were measured using a Tecan Infinite M1000 plate reader. Avoid multiple freeze/thaw cycles.
GenBank	NM_003679
Mw(kda)	58 kDa

Synonyms	Kynurenine 3-Monooxygenase, Kynurenine 3-Hydroxylase, and EC 1.14.13.9
Uniprot	O15229

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Zwilling, D., et al., Cell. 2011; 6(10):863-874. 2. Giorgini, G., et al., Nature Genetics. 2005; 37: 526-531.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200009	11307	100 ug	$352		Add to Cart

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