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Home / Proteins & Peptide

KEAP1, His-tag

SKU : BHP18201520

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BPS Bioscience

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Mfr.No.	70040-1
Description	Human Keap1, also known as Kelch-likeECH-associated protein, INRF2, KIAA0132,and KLHL19, GenBank Accession No.NM_203500, a.a. 321-609 expressed in anE. coli cell expression system. MW = 32.6kDa
Immunogen Region	321-609
Tag	His-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 200 mMimidazole, 3 mM DTT, 20% glycerol
Species	Human
Application	Useful for studying protein binding and screening small molecular inhibitors
Notes	Binding of Keap1 to Nrf2 was measured using BPS Keap1:Nrf2 Assay Kit, Cat. #72020. Briefly, varying concentrations of Keap1 were added to wells containing FITC-labeled Nrf2 peptide in assay buffer and incubated for 30 min. at room temp. Fluorescence was measured at ex 485nm, em 530 nm using a Bio-Tek fluorescent microplate reader. Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_203500
Mw(kda)	32.6 kDa

Synonyms	Kelch-like ECH-associated protein, INRF2, KIAA0132, KLHL19, Nrf
Uniprot	Q14145

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Dinkova-Kostova, A.T., et al., 2002. Proc. Nat. Acad. Sci. (USA). 99(18): 11908-11913. 2. Inoyama, D., et al. 2012. J. Biomol. Screening 17(4): 435-447.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201520	70040-1	100 ug	$561		Add to Cart

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