Human Keap1, also known as Kelch-likeECH-associated protein, INRF2, KIAA0132,and KLHL19, GenBank Accession No.NM_203500, a.a. 321-609 expressed in anE. coli cell expression system. MW = 32.6kDa
Immunogen Region
321-609
Tag
His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mMimidazole, 3 mM DTT, 20% glycerol
Species
Human
Application
Useful for studying protein binding and screening small molecular inhibitors
Notes
Binding of Keap1 to Nrf2 was measuredusing BPS Keap1:Nrf2 Assay Kit, Cat.#72020. Briefly, varying concentrations ofKeap1 were added to wells containingFITC-labeled Nrf2 peptide in assay bufferand incubated for 30 min. at room temp.Fluorescence was measured at ex 485nm,em 530 nm using a Bio-Tek fluorescentmicroplate reader.
Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
1. Dinkova-Kostova, A.T., et al., 2002. Proc. Nat. Acad. Sci. (USA). 99(18): 11908-11913. 2. Inoyama, D., et al. 2012. J. Biomol. Screening 17(4): 435-447.