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Home / Proteins & Peptide

JMJD2E (KDM4DL), GST-tag

SKU : BHP18200342

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BPS Bioscience

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Mfr.No.	50118
Description	Human JMJDE , also known as KDM4DL (GenBank Accession No. NM_001161630), a.a. 2-337 with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells using a Baculovirus expression system. Host cell line: Sf9 cells Specific activity: 0.14 pmole/min/ug
Immunogen Region	2-337
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JMJD2E (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_001161630
Mw(kda)	65 kDa

Synonyms	KDM4DL, JMJDE, KDM4D-Like
Uniprot	B2RXH2

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Fodor, B.D., et al., (2006). Genes Dev. 20(12): 1557-1562. 2. Beyer, S., et al., (2008). J. Biol. Chem. 283(52):36542-52.Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200342	50118	100 ug	$511.50		Add to Cart

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