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Home / Proteins & Peptide

JMJD2D (KDM4D), GST-tag

SKU : BHP18200341

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BPS Bioscience

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Mfr.No.	50117
Description	Human JMJD2D (GenBank Accession No. NM_018039), amino acids 2-354, with N-terminal GST tag, MW=67 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: >= 0.014 pmole/min/ug
Immunogen Region	2-354
Tag	N-terminal GST-tag
Formulation	40 mM Tris pH 8.0, 110mM NaCl, 2.2 mM KCl, 3 mM DTT and20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1 mM TCEP, 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JMJD2D (5 - 25 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_018039
Mw(kda)	67 kDa

Synonyms	KDM4D
Uniprot	Q6B0I6

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Katoh, M., Int J Oncol. 2004 Jun; 24(6):1623-8. 2. Shin, S., et al., Biochem Biophys Res Commun. 2007 Feb 23; 353(4):973-7.Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200341	50117	100 ug	$484		Add to Cart

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