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Home / Proteins & Peptide

JMJD2C(KDM4C), GST-tag

SKU : BHP18200335

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BPS Bioscience

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Mfr.No.	50105
Description	Human JMJD2C (GenBank Accession No. BC143571) (a.a. 2-372) with N-terminal GST-tag, MW=69 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 0.17 pmole/min/ug
Immunogen Region	2-372
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JMJD2C (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	BC143571
Mw(kda)	69 kDa

Synonyms	KDM4C, GASC1
Uniprot	Q9H3R0

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Vinatzer, U., et al., Clin Cancer Res. 2008 Oct 15;14(20):6426-31. 2. Pollard, P.J., et al., Biochem J. 2008 Dec 15;416(3):387-94.Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200335	50105	100 ug	$511.50		Add to Cart

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