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Home / Proteins & Peptide

JMJD2B (KDM4B), GST-tag

SKU : BHP18200337

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BPS Bioscience

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Mfr.No.	50111
Description	Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), a.a. 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system. Host cell line: Sf9 cells Specific activity: >=2.9 pmol/min/ug.
Immunogen Region	2-500)
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mm KCl, 10% glycerol, 18 mM glutathione, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay done using Alphascreen technology, using BPS JMJD2B Homogeneous Assay Kit, # 50414. JMJD2B enzyme (60 ng/ul) is incubated with biotinylated histone H3 peptide substrate in JMJ assay buffer in a 10 ul reaction (50 mM HEPES (pH 8.0), 1 mM alpha-ketoglutarate, 80 uM FeSO4, 2 mM ascorbic acid) for 1-2 hours at RT. AlphaLISA anti-Mouse Acceptor beads (PerkinElmer) are added, followed by the primary antibody addition and 30 min incubation at RT. Alphascreen Streptavidin-conjugated donor beads (PerkinElmer) are added. After 10-15 minute incubation at room temperature, Alphacounts are measured. Avoid freeze/thaw cycles.
GenBank	NM_015015
Mw(kda)	83 kDa

Synonyms	JHDM3B, KDM4B
Uniprot	O94953

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Fodor, B.D., et al. (2006). Genes Dev. 20(12): 1557-1562. 2. Beyer S., et al. (2008). J. Biol. Chem. 283(52):36542-52.Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200337	50111	100 ug	$511.50		Add to Cart

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