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Home / Proteins & Peptide

JMJD2B(KDM4B) , FLAG-tag

SKU : BHP18200334

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BPS Bioscience

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Mfr.No.	50104
Description	Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015) (a.a. 1-500) with C-terminal FLAG-tag, MW=57 kDa, expressed in Sf9 cells using a Baculovirus expression system. Host cell line: Sf9 cells Specific activity: 0.09 pmole/min/ug
Immunogen Region	1-500
Tag	C-terminal FLAG-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JMJD2B (20 - 100 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_015015
Mw(kda)	57 kDa

Synonyms	JHDM3B, KDM4B
Uniprot	O94953

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Fodor, B.D., et al. (2006). Genes Dev. 20(12): 1557-1562. 2. Beyer S., et al. (2008). J. Biol. Chem. 283(52):36542-52.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200334	50104	100 ug	$511.50		Add to Cart

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