Human JMJD2A, also known as JHDM3A and KDM4A (GenBank Accession No. NM_014663) (a.a. 1-350) with N-terminal His-tag, MW=42 kDa, expressed in an E. coli expression system.
Specific activity: 0.084 pmole/min/ug
Immunogen Region
1-350
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JMJD2A (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.