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Home / Proteins & Peptide

JMJD1B (KDM3B), FLAG-tag

SKU : BHP18200399

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BPS Bioscience

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Mfr.No.	50421
Description	Human JMJD1B, also knownas lysine-specific demethylase 3B(KDM3B), JHDM2B, and KIA1082,GenBank Accession No. NM_016604/NP_057688, a.a. 2-1761 (end) with NterminalAvi- and FLAG-tags, MW=195kDa, expressed in a baculovirus-infectedSf9 cell expression system. Host cell line: Sf9 cells Specific activity: 0.0045 pmole/min/ug
Immunogen Region	2-1761 (end)
Tag	N-terminal Avi-, FLAG-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction wasperformed with 20 mM HEPES (pH 7.4),150 mM NaCl, 500 uM -ketoglutarate, 25uM iron, 2 mM ascorbic acid, 0.01%Tween-20, 0.5 uM biotinylated peptidesubstrate, and JMJD1B added to thewells. Add antibody against methylatedH3K9. Incubate for 30 min. followed byAlphascreen detection. Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_016604
Mw(kda)	195 kDa

Synonyms	KDM3B, JHDM2B, KIA1082
Uniprot	Q7LBC6

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Kim, J-Y, et al., Mol. Cel. Biol. 2012. 32(14):2917-2933. 2. Mikhaleva, I.I., et al., Peptides. 2011. 32(4): 826-831.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200399	50421	20 ug	$511.50		Add to Cart

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