Human JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, GenBank Accession No. NM_004187, full length, a.a. 2-1560(end), with N-terminal FLAG- and His-tags, MW= 181 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 0.078 pmole/min/ug
Immunogen Region
2-1560(end)
Tag
N-terminal FLAG-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ug/ml FLAG peptide
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JARID1C (50 - 200 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Avoid freeze/thaw cycles.
GenBank
NM_004187
Mw(kda)
181 kDa
Synonyms
SMCX, Smcy homolog, X-linked, KDM5C, XE169
Uniprot
P41229
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Xu, J., et al. (2008). PLoS One 3(7):e2553 2. Iwase, S., et al. (2007). Cell 128(6):1077-88. 3. Jenson, L.R., et al. (2005). Am. J. Hum. Genet. 76(2):227-36.