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Home / Proteins & Peptide

JARID1B (KDM5B, PLU-1), His-FLAG-tags

SKU : BHP18200345

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BPS Bioscience

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Mfr.No.	50121
Description	Human JARID1B, also known as PLU-1 and KDM5B (GenBank Accession No. NM_006618), a.a. 2-751 with N-terminal His-FLAG-tag, MW=88 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 0.22 pmole/min/ug
Background	Histone demethylase that demethylates Lys-4 of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 Lys-9 or H3 Lys-27. Demethylates trimethylated, dimethylated and monomethylated H3 Lys-4. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
Immunogen Region	2-751
Tag	N-terminal His-FLAG-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 80 ug/mL FLAG peptide and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl 500 uM alpha-ketoglutarate, 25 uM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JARID1B (20 - 100 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_006618
Mw(kda)	88 kDa

Synonyms	JARID1B, PLU-1, KDM5B, Retinoblastoma-Binding Protein 2 Homolog 1, RBP2-H1, CT31, Cancer/Testis Antigen 31, PLU-1, Jumonji, AT Rich Interactive Domain 1B, Lysine-Specific Demethylase 5B
Uniprot	Q9UGL1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Yamane K, et al. (2007). Mol. Cell 25(6): 801-12. 2. Barrett A, et al. (2007). Int. J. Cancer 121(2): 265-75.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200345	50121	20 ug	$511.50		Add to Cart

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