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Home / Proteins & Peptide

JARID1A (KDM5A), His-Avi-tags, FLAG-tag

SKU : BHP18200358

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BPS Bioscience

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Mfr.No.	50155
Description	Human JARID1A, also known as KDM5A and RBBP2, GenBank Accession No. NM_001042603, a.a. 2-1090 with N-terminal His-Avi-tags and C-terminal FLAG-tag, MW= 128 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: >=0.024 pmol/min/ug
Immunogen Region	2-1090
Tag	N-terminal His-Avi-tags, C-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 80 ug/ml FLAG peptide, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay conditions: 10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 500 uM alpha-ketoglutarate, 25 uM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JARID1A (80 - 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_001042603
Mw(kda)	128 kDa

Synonyms	KDM5A, RBBP2, JARID1A
Uniprot	P29375

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Roesch, A., et al. (2005). Mod Pathol. 18(9):1249-57. 2. Zhou X., et al. (2010). Cancer Res. 70(10):4214-21 3. van Zutven LJ, et al. (2006). Genes Chromosomes Cancer 45(5):437-46.Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200358	50155	20 ug	$522.50		Add to Cart

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