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Home / Proteins & Peptide

JARID1A (KDM5A), FLAG-tag

SKU : BHP18200336

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BPS Bioscience

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Mfr.No.	50110
Description	Human JARID1A, also known as KDM5A and RBBP2, GenBank Accession #NM_001042603), a.a. 1-1090 with C-terminal FLAG-tag, MW= 125 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 0.027 pmole/min/ug
Immunogen Region	1-1090
Tag	C-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JARID1A (80 - 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_001042603
Mw(kda)	125 kDa

Synonyms	KDM5A, RBBP2, JARID1A
Uniprot	P29375

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Roesch, A., et al., (2005). Mod Pathol. 18(9):1249-57. 2. Zhou, X., et al., (2010). Cancer Res. 70(10):4214-21 3. Van Zutven, LJ., et al., (2006). Genes Chromosomes Cancer 45(5):437-46.Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200336	50110	20 ug	$550		Add to Cart

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