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Home / Proteins & Peptide

Jak3, His-tag

SKU : BHP18200249

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Mfr.No.	40452
Description	Human Jak3 (Janus kinase 3), also known as LJAK, GenBank Accession No. NM_000215, a.a. 811-1124(end) with N-terminal His-tag, MW= 37 kDa, expressed in a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 500 pmol/min/ug.
Immunogen Region	811-1124(end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	40 mM Tris (pH 7.4), 10 mM MgCl₂, 0.1 mg/ml BSA, 100 uM ATP, and 500 uM Jaktide as a substrate at 30°C for 20 min. ADP-Glo reagents (Promega) are used to measure the amount of ATP transferred during the catalysis. Avoid freeze/thaw cycles.
GenBank	NM_000215
Mw(kda)	37 kDa

Synonyms	JAK3, Janus kinase-3, Just Another Kinase 3, LJAK
Uniprot	P52333

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Kim S. et al., Cell. Signal. 21 (6), 892-898 (2009). 2. Mullighan C.G. et al., Proc. Natl. Acad. Sci. U.S.A. 106 (23), 9414-9418 (2009).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200249	40452	20 ug	$412.50		Add to Cart

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