Human Jak2 with V617F mutation, GenBank Accession No. NM_004972, a.a. 513-1132 (end), with N-terminal GST tag, C-terminal Avi-Tag, expressed in a HEK293 cell expression system and enzymatically Biotin-labeled using AviTag technology.
Immunogen Region
513-1132 (end)
Tag
h N-terminal GST tag, C-terminal Avi-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20mM GSH and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Concentration
0.2 mg/ml
Notes
Avoid freeze/thaw cycles.
GenBank
NM_004972
Mw(kda)
100 kDa
Synonyms
JAK2, Janus kinase-2, Just Another Kinase 2, V617F
Uniprot
O60674
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Reference
1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844. 2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.