Human Jak2 (GenBank Accession No. NM_004972), containing the catalytic and the pseudo kinase domain (a.a. 532-1132) with N-terminal His-GST-tag, MW=98 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 8 pmol/min/ug
Immunogen Region
532-1132
Tag
N-terminal His-GST-tags
Formulation
45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.4 mM KCl, 0.04% Tween-20, 3 mM DTT, 10% glycerol and 250 mM Imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, 2 uM substrate (Tyr Peptide 6, Z-lyte kinase assay kit from Invitrogen), and 0-100 ng enzyme at 37°C for 1 hour.