Human Jak2 containing the catalytic and the pseudo kinase domain (a.a. 532-1132), also known as Janus kinase-2, Just Another Kinase 2, Jak-2-JH1, JH2, GenBank Accession No. NM_004972, with N-terminal GST-Tag and C-terminal Avi-TagTM co-expressed with PTP1B in HEK293 expression system. This protein is enzymatically Biotin-labeled using AviTag technology. Percentage of phosphorylation<= 10%. MW=100 kDa.
Immunogen Region
532-1132
Tag
N-terminal GST-Tag, C-terminal Avi-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20 mM Imidazole and 20% glycerol.
Species
Human
Notes
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_004972
Mw(kda)
100 kDa
Synonyms
Janus kinase-2, Just Another Kinase 2, Jak-2-JH1, JH2
Uniprot
O60674
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.