Human Jak2 (GenBank Accession No. NM_004972), (a.a. 513-827) with C-terminal His-Avi-tag, MW=39 kDa, expressed in an Sf9 expression system. This protein was biotinylated using AviTag technology.
Immunogen Region
513-827
Tag
C-terminal His-Avi-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 230 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 0.2mM TCEP, 20% glycerol and additional 400mM imidazole
Species
Human
Application
Useful as a positive control for Western blot studies.
Concentration
0.154 mg/ml
Notes
Avoid freeze/thaw cycles.
GenBank
NM_004972
Mw(kda)
39 kDa
Synonyms
JAK2, Janus kinase-2, Just Another Kinase 2, JAK2-JH2
Uniprot
O60674
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution.
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844. 2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.