Human Jak2, also known as Janus kinase-2, Just Another Kinase 2, JAK2-JH2, GenBank Accession No. NM_004972, a.a. 1-1132 (end), with C-terminal FLAG-Avi-Tag, co-expressed with PTP1B in HEK293 expression system. This protein is enzymatically Biotin-labeled using AviTag technology. Percentage of phosphorylation = 4%. MW=134 kDa.
Immunogen Region
1-1132
Tag
C-terminal FLAG-Avi-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 100ug/ml FLAG peptide and 20% glycerol.
Species
Human
Notes
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_004972
Mw(kda)
134 kDa
Synonyms
Janus kinase-2, Just Another Kinase 2, JAK2-JH2
Uniprot
O60674
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.