Human IRAK4 (GenBank Accession No. NM_001114182), a.a. 2-end with N-terminal GST-tag, MW= 77.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 25 pmol/min/ug
Immunogen Region
2-end
Tag
N-terminal GST-tag
Formulation
45 mM Tris-HCl, pH 8.0, 425 mM NaCl, 2.5 mM KCl, 3 mM DTT, and 10% Glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35 and 2 uM substrate (Invitrogen ZLyte SER/THR 7) at room temperature for 1 hour.