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Home / Proteins & Peptide

IRAK1, His-tag

SKU : BHP18200191

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Mfr.No.	40202
Description	Human interleukin-1 receptor-associated kinase 1 (IRAK1), GenBank Accession No. NM_001569, a.a 186-530, with N-terminal His-tag, MW = 40 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: >=43 pmol/min/ug
Immunogen Region	186-530
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, variable amounts of imidazole, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 10 mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent Avoid freeze/thaw cycles.
GenBank	NM_001569
Mw(kda)	40 kDa

Uniprot	P51617

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%)

Reference	1. Ku C.L. et al., J. Exp. Med. 2007; 204(10): 2407-2422.2. Hoarau C. et al., J. Immunol. 2007; 179(7): 4754-4765.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200191	40202	10 ug	$401.50		Add to Cart

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