Human insulin receptor (INSR) (GenBank Accession No. NM_001079817), a.a. 999-end with N-terminal GST-tag, MW=67 kDa, expressed in a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 43.3 pmol/min/ug
Immunogen Region
999-end
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, autophosphorylation and phosphorylation of target substrates, screening inhibitors, and selectivity profiling.
Notes
The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 uM of ATP. Substrate : 4 uM Tyr peptide 1 from Invitrogen.
Avoid freeze/thaw cycles.
GenBank
NM_001079817
Mw(kda)
67 kDa
Synonyms
INSR, insulin receptor
Uniprot
P06213
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Zhou, P. et al. J. Pediatr. Endocrinol. Metab. 21 (4), 369-375 (2008). 2. Machorro-Lazo, M.V. et al., Gynecol. Obstet. Invest. 67 (1), 14-19 (2008).