Papain is a nonspecific, cysteine-endopeptidase isolated from papaya latex that is used in a wide variety of applications. When IgG molecules are incubated with papain in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing three fragments of similar size: two Fab fragments and one Fc fragment. The Fc fragments can be separated from the Fab fragments using immobilized Protein A or by ion exchange chromatography.
Source
Purified from Papaya latex
Form
Liquid
Protein Name
Immobilized Papain
Bioactivity
Not test
Research Area
Cell Biology
Purity
>95% (SDS-PAGE)
Storage
Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.