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Home / Proteins & Peptide

IL-1beta, Porcine

SKU : BHP18500419

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iXCells Biotechnologies

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Mfr.No.	IX03278, IX03278-1, IX03278-10, IX03278-50
Description	Interleukin-1 beta (rpIL-1beta) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1alpha and IL-1beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. While IL-1alpha and IL-1beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1beta stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity.Recombinant Porcine IL-1beta produced in E. coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rpIL-1beta has a molecular mass of 17.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	17.7 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Accession Number	P26889
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	ED50 1ng/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0x 106 IU/mg.

Synonyms	Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Sequence	Ala115-Pro267 (Accession #:P26889), expressed with an N-terminal Met

Purity	95% as analyzed by SDS-PAGE.
Storage	Lyophilized recombinant Porcine IL-1beta, remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Porcine IL-1beta should be stable up to 1 week at 4°C or up to 3 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500419-1	IX03278-1	1mg	$2185		Add to Cart
BHP18500419-2	IX03278-10	10ug	$120		Add to Cart
BHP18500419-3	IX03278-50	50ug	$245		Add to Cart

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