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Home / Proteins & Peptide

IL-18, Rat

SKU : BHP18500223

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iXCells Biotechnologies

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Mfr.No.	IX03025, IX03025-1, IX03025-10, IX03025-50
Description	Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.Recombinant rat Interleukin-18 (rrIL-18) produced in E. coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rrIL-18 has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
Reconstitution	Reconstituted in ddH2O at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH8.0.
Molecular Weight	18.4 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Rat
Accession Number	P97636-1
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	ED50 2ug/ml resulted in the secretion of 0.15 ng/ml h-IFN-gamma when tested using KG-1 cell line, corresponding to a specific activity of 500 units/mg.

Synonyms	NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Interferon-gamma-inducing factor, IGIF, IL-1g, IL1F4, IL-1 gamma
Sequence	MHFGRLHCTT AVIRSINDQV LFVDKRNPPV FEDMPDIDRTANESQTRLII YMYKDSEVRG LAVTLSVKDG RMSTLSCKNKIISFEEMNPP ENIDDIKSDL IFFQKRVPGH NKMEFESSLYEGHFLACQKE DDAFKLVLKR KDENGDKSVM FTLTNLHQS

Purity	95% by SDS-PAGE analyses.
Storage	Lyophilized recombinant ratInterleukin-18 (rrIL-18) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrIL-18 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500223-1	IX03025-1	1mg	$3993		Add to Cart
BHP18500223-2	IX03025-10	10ug	$107		Add to Cart
BHP18500223-3	IX03025-50	50ug	$563		Add to Cart

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