Human secreted interleukin17 A (IL-17A) also known as, IL-17 andcytotoxic T lymphocyte antigen 8 (CTLA8)GenBank Accession No. NM_ 002190, a.a20-155(end), expressed in an e. coliexpression system. This protein existsnaturally as a homodimer; M.W. = 17.5 kDa(monomer); 35 kDa dimer
Host cell line: E. coli
Immunogen Region
20-155(end)
Formulation
10 mM sodium phosphate, pH 6.0, and 220 mM NaCl
Species
Human
Application
Useful for studying protein function and protein-protein interaction. studies.
Notes
100 ng of IL-17A wascoated onto a 96-well plate overnight. Theindicated amount of IL-17RA (BPS Cat.#91013), Biotin was added in IL-17 AssayBuffer and allowed to incubate at roomtemperature for 2 hr. Strep-HRP was added,followed by chemiluminescent substrate anddetection.
The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 g/ml in PBS + glycerol and stored at -80°C.