Human IKKbeta, also known as IKK2, IKBKB, IKKB, and NFKBIKB. GenBank Accession No. NM_001556, full-lengthexpressed in a baculovirus-infected Sf9 insect cell expression system, MW = 105 kDa.
Host cell line: Sf9 cells
Specific activity: 40 pmol/min/ug
Immunogen Region
Full Length
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 3 mM DTTand 20% glycerol.
Species
Human
Application
Useful for studying enzyme kinetics and screening small molecule inhibitors.
Notes
Assay was done in a Kinase buffer using IKKtide as a substrate with 20 M ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank
NM_001556
Mw(kda)
105 kDa
Synonyms
Inhibitor of kappaB kinase beta, IKK2, IKBKB, IKKB, and NFKBIKB
Uniprot
Q14920
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Woronicz, J.D. et al., Science. 1997; 278: 866-869. 2. Yin, M.J., et al., Nature. 1998; 396: 77-80.