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Home / Proteins & Peptide

IGFBP7, Avi-His-Tag, Biotin-Labeled Recombinant-25 ug

SKU : BHP18201480

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BPS Bioscience

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Mfr.No.	101546-1
Description	Recombinant human IGFBP7 (insulin like growth factor binding protein 7), encompassing amino acids 27-282(end). This construct contains a C-terminal Avi-Tag followed by a His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag and affinity purified. host species: HEK293 Host species: HEK293
Immunogen Region	27-282(end)
Tag	C-terminal Avi-His Tag
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	CD93 protein (Biohippo #101543) was coated onto a 96-well plate overnight at 4°C (50 ul/well at a concentration of 4 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (Biohippo #79311) and blocked using 100 ul of Blocking Buffer 2 (Biohippo #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified biotinylated IGFBP7 protein (Biohippo #101546), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values. Avoid freeze/thaw cycles.
GenBank	NM_001553
Mw(kda)	30 kDa + glycans

Synonyms	Insulin-like growth factor-binding protein 7, IBP-7, IGF-binding protein 7, IGFBP-7, IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, Prostacyclin-stimulating factor, Tumor-derived adhesion factor (TAF), MAC25, PSF
Uniprot	Q16270

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201480	101546-1	25 ug	$324.50		Add to Cart

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