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Home / Proteins & Peptide

IFN-alpha 1b, Human

SKU : BHP18500115

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iXCells Biotechnologies

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Mfr.No.	IX02866, IX02866-10, IX02866-2
Description	At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20 °C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, containing 4 % mannitol and 1 % HSA.
Molecular Weight	Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 167 amino acids.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	Human
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Endotoxin Level	Less than 1 EU/ug of rHuIFN-alpha1b as determined by LAL method.
Bioactivity	Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 x 108 IU/mg.

Synonyms	Interferon-?1b (IFN-?1b), Human;
Sequence	MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFPQEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDEDLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNVDSILAVKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQERLRRKE

Purity	96 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500115-1	IX02866-10	10ug	$170		Add to Cart
BHP18500115-2	IX02866-2	2ug	$74		Add to Cart

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