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Home / Proteins & Peptide

IDH2 (R140Q), FLAG-tag

SKU : BHP18201533

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Mfr.No.	71100-2
Background	Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). The IDH2 homodimer is an the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production and may tightly associate or interact with the pyruvate dehydrogenase complex.
Immunogen Region	1-452(end)
Tag	C-terminal FLAG-tag
Description	Human isocitrate dehydrogenase 2, also known as IDH2, GenBank Accession No. NM_002168, a.a. 1-452(end) with with Arg to Gln mutation on a.a. 140 and C-terminal FLAG-tag, MW = 52 kDa, expressed in a baculovirus infected Sf9 cell expression system. Specific activity: >= 61 pmol/min/ug
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/ml FLAG peptide, 0.04% Tween-20, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	IDH reductive activity was measured in 200 ul reaction containing 25 mM Tris (pH 7.4), 150 mM NaCl, 10 mM MgCl₂, 0.03% BSA, 1 mM alpha-Ketoglutarate, 10 uM NADPH and IDH. Depletion of NADPH was monitored continuously at Abs340 nm for 20 min. Molar extinction coefficient of NADPH is 6,200 M^-1cm^-1. This protein runs aberrantly by SDS-PAGE. Avoid freeze/thaw cycles.
GenBank	NM_002168
Mw(kda)	52 kDa

Synonyms	Isocitrate Dehydrogenase 2, IDH2, oxalosuccinate decarboxylase, IDHM
Uniprot	P48735

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Schaap, F.G., et al., Adv. Anat. Pathol. 2013;20(1):32-38. 2. Grassian, A.R., et al., J. Biol. Chem. 2012;287(50):42180-42194.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201533	71100-2	100 ug	$555.50		Add to Cart

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