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Home / Proteins & Peptide

IDH2, FLAG-tag

SKU : BHP18201526

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Mfr.No.	71074-1
Description	Human isocitrate dehydrogenase 2, also known as IDH2, (GenBank Accession No. NM_002168), a.a. 1-452(end) with C-terminal FLAG-tag, MW = 52 kDa, expressed in a baculovirus infected Sf9 cell expression system. Specific activity: >=14,200 pmol/min/ug.
Immunogen Region	1-452(end)
Tag	C-terminal FLAG-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAGpeptide, 3 mM DTT and 10% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The reaction mixture contained 50 mM Tris, pH 7.4, 5 mM MgCl₂, 200 uM DL-isocitrate, 200 uM NADP+, and various amount of IDH2 in a volume of 200 uL. Reactions were initiated with the addition of IDH2, and the increase in NADPH absorbance at 340 nm was continuously monitored for 7 min. Molar extinction coefficient of NADPH is 6,220 M^-1cm^-1. Avoid freeze/thaw cycles.
GenBank	NM_002168
Mw(kda)	52 kDa

Synonyms	Isocitrate Dehydrogenase 2, IDH2, oxalosuccinate decarboxylase
Uniprot	P48735

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Schaap, F.G., et al., Adv Anat Pathol 2013;20(1):32-38. 2. Grassian, A.R., et al., J Biol Chem 2012;287(50):42180-42194.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201526	71074-1	50 ug	$319		Add to Cart

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