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Home / Proteins & Peptide

IDH1, FLAG-tag

SKU : BHP18201528

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BPS Bioscience

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Mfr.No.	71075-1
Immunogen Region	1-414(end)
Tag	C-terminal FLAG-tag
Description	Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, GenBank Accession No. NM_005896, full length (a.a. 1-414(end)) with C-terminal FLAG-tag, MW= 47.6 kDa, expressed in Sf9 cells via a baculovirus expression system. Specific activity: >=5600 pmol/min/ug.
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAG peptide, 3 mM DTT and 10% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was conductedin 50 mM Tris (pH 7.4), 10 mM MgCl₂, 200uM DL-isocitrate, and 200 uM NADP.Incubate for 30 minutes at roomtemperature. Read at Abs340 using TecanInfinite 1000 (5 flash/10 ms settle time). Avoid freeze/thaw cycles.
GenBank	NM_005896
Mw(kda)	47.6 kDa

Synonyms	Isocitrate Dehydrogenase 1, IDH1, oxalosuccinate decarboxylase
Uniprot	O75874

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Dang, L., et al. (2010). Trends Mol. Med. 16(9):387-97. 2. Paschka, P., et al. (2010). J. Clin. Oncol. 28(22):3636-43. 3. Fu, Y., et al. (2010). Biochem. Biophys. Res. Commun. 397(2):127-30.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201528	71075-1	50 ug	$319		Add to Cart

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