Human Ras Binding Domain (RBD) of Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 50-140), expressed with an N-terminal 6XHis and C-terminal FLAG in E. coli. Specifically binds to and precipitates Ras-GTP from cell lysate. Can be bound to glutathione coated plates prior to precipitating Ras-GTP.
Product Type
Recombinant Proteins
Tag
His-FLAG
Field of Use
Drug Targets
Molecular Weight
14.44 kDa
Species
Human
Application
ELISA, Western Blot, Inhibitor Screening, binding assays, enzyme kinetics, and selectivity profiling.
Expression Source
E. coli
Protein Length
90 aa
Uniprot
P04049
Shipping
Dry Ice
Purity
>95%, SDS-PAGE
Storage
Stable for 6 months at -80°C, Avoid freeze/thaw cycles