Human IDO2, also known as Indoleamine 2,3-dioxygenase 2, GenBank Accession No. NM_194294, a.a. 15-420(end) with an N-terminal His-tag, expressed in the presence of hemin in an E. coli cell expression system. MW = 46 kDa.
Immunogen Region
15-420(end)
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT and 150 mMimidazole.
Species
Human
Application
Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Notes
IDO2 activity was confirmed using the IDO2 Inhibitor Screening Assay Kit (BPS Cat. #72022) and following the protocol provided.
Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended, if necessary, usecarrier protein (BSA 0.1 - 0.5%).
GenBank
NM_194294
Mw(kda)
46 kDa
Synonyms
Indoleamine 2,3-dioxygenase 2, IDO-2, IDO
Uniprot
Q6ZQW0
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Metz, R., et al., Canc. Res. 2007; 67(15): 7082-7087. 2. Witkiewicz, A.K., et al., J. Am. Coll. Surgeons. 2009; 208(5): 781-787. 3. Flick, H.E., et al., Int. Nat. J. Tryptophan Res. 2013; 6: 35-45.