Human IDO1, also known as Indoleamine 2,3-dioxygenase 1, GenBank Accession No. NM_002164, a.a. 2-403(end) with an N-terminal His-tag, expressed in the presence of hemin in an E. coli cell expression system. MW = 46 kDa.
Immunogen Region
2-403(end)
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Species
Human
Application
Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Notes
IDO1 activity was confirmed using the IDO1 Inhibitor Screening Assay Kit (BPS Cat. #72021) and following the protocol provided with the kit.
Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank
NM_002146
Mw(kda)
46 kDa
Synonyms
Indoleamine 2,3-dioxygenase 1
Uniprot
P14902
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Lob, S. et al., Cancer Immunol. Immunother. 2009; 58(1): 153-157. 2. Liu, X. et al., Blood. 2010; 115(17): 3520-3530. 3. Flick, H.E., et al., Int. Nat. J. of Tryptophan Res. 2013; 6: 35-45.