Human CD46-BC, also known as membrane co-factor protein, Genbank Accession No. BC_030594, a.a. 35-328 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 36.6 kDa (precursor), 34.2 kDa (mature). Note: this protein is expressed with a 34 amino acid signal sequence that is cleaved after secretion and is heavily glycosylated, resulting in a protein that runs at a higher apparent MW.
Immunogen Region
35-328
Tag
C-terminal His-tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Species
Human
Application
Useful for studies of protein function and protein-protein interaction.
Notes
This protein isexpressed with a 34 amino acid signalsequence that is cleaved after secretionand is heavily glycosylated, resulting in aprotein that runs at a higher apparentMW. The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
BC_030594
Mw(kda)
34.2 kDa+glycans
Synonyms
membrane co-factor protein
Uniprot
P15529
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Liszewski, MK., Atkinson, JP., J. Immunol. 1996; 156(11):4415-4421. 2. Gerlier, D., et al., Gen. Virol. 1994. 75(Pt 9):2162-2171.