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Home / Proteins & Peptide

Human CD226, Fc fusion, Biotin-labeled

SKU : BHP18201622

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Mfr.No.	71253-2
Description	Human CD226 antigen also known as DNAX accessory molecule 1 (DNAM-1), GenBank Accession No. NM_006566, a.a. 19-247 fused to Fc region of human IgG1, this protein is enzymatically biotinylated using AviTag technology and expressed in a HEK293 cell expression system. MW = 54.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region	19-247
Tag	C-terminal Fc fusion (Human IgG1), AviTag
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Species	Human
Application	Useful for studying protein binding and for screening small molecules and antibodies.
Notes	CD226, biotin binding to CD155, His-tag was confirmed according to the protocol for BPS Cat. 72052. Briefly, 6 ng of CD226-biotin was mixed with varying amounts of CD155, His-tag in CD226 assay buffer at a final volume of 10 ul and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 min, followed by Strep-conjugated acceptor beads for 30 min and reading alpha-counts. The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank	NM_006566
Mw(kda)	54.7 kDa

Synonyms	DNAX accessory molecule 1 (DNAM-1)
Uniprot	Q15762

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Bottino, C., et al., J. Exp. Med. 2003; 198(4): 557-567. 2. Hafler, J., et al., Genes Immun. 2009; 10: 5-10.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201622	71253-2	50 ug	$478.50		Add to Cart

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