JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

HSP90alpha, His-tag

SKU : BHP18200382

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	50290
Description	Human Heat Shock Protein 90alpha (GenBank Accession No. NM_005348), full length with C-terminal His-tag, MW = 85.5 kDa, expressed in an E. coli expression system. Specific activity: Kd = 11.8 nM.
Background	Hsp90 (90 kDa heat shock protein) is a molecular chaperone that aids protein folding and quality control for a large number of client proteins. Functional Hsp90 operates as dimer and has intrinsic ATPase activity. The Hsp90 dimer acts in concert with other chaperones (e.g. Hsp70) and is regulated by a number of co-chaperones/accessory proteins (e.g. Hop, cdc37). Hsp90 has been shown to interact with> 100 proteins and some notable clients include kinases (e.g. Raf-1), nuclear hormone receptors (e.g. estrogen receptors), transcription factors (e.g. p53), GPCRs (e.g. CB2 receptors) and ion channels (e.g. CFTR). In humans, the Hsp90beta isoform is constitutively expressed whereas the Hsp90alpha isoforms is expressed under stress conditions. Hsp90 plays an important role in some tumor cell types by stabilising mutated oncogenic proteins.
Immunogen Region	full length
Tag	C-terminal His-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Buffer contains 0.1M HEPES, pH 7.4, 0.25M MgCl2, 0.25M KCl, 0.1M Na2MoO4, and 0.05% Triton X. Incubated Hsp90alpha in a series dilution with 5 nM FITC-labeled geldanamycin for 150 minutes at room temperature. Fluorescence polarization measured at ex 470/em528. Avoid freeze/thaw cycles.
GenBank	NM_005348
Mw(kda)	85.5 kDa

Synonyms	HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90alpha
Uniprot	P07900

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Wayne N. and Bolon D.N. J Biol Chem. 2007 Nov 30;282(48):35386-95. 2. Pearl L.H., et al. Biochem J. 2008 Mar 15;410(3):439-53.Application Reference(s): 1. The quinone methide aurin is a heat shock response inducer that causes proteotoxic stress and Noxa-dependent apoptosis in malignant melanoma cells (2015) 2.Herbimycins D-F, ansamycin analogues from Streptomyces sp. RM-7-15 (2013)

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18200382	50290	200 ug	$478.50		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.