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Home / Proteins & Peptide

HRV 3C Protease, His-GST-tags

SKU : BHP18200394

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BPS Bioscience

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Mfr.No.	50319
Description	Human rhinovirus 3Cprotease (HRV 3C Protease) with NterminalHis-GST-tags (can be easilyremoved by either Ni-chelating orGlutathione (GSH) resin along with thecleaved tag.), Recognizes the cleavagesite of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro.Cleaves between Gln and Gly. MW = 47kDa in E. coli expression system. Specific activity: 1 Unit of HRV 3C Protease cleaves>95% of 100 ug of target protein at 4°C for 16 hours. Unit definition: 1 Unit of HRV 3C Protease cleaves>95% of 100 ug of target protein at 4°C for 16 hours.
Tag	N-terminal His-GST-tags
Formulation	50 mM Tris-HCl, pH 8.0,150 mM NaCl, 1 mM EDTA, 1 mM TCEP,50% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with HRV3C Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 14 mM beta-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa. Avoid freeze/thaw cycles.
Mw(kda)	47 kDa

Synonyms	Human rhinovirus 3C protease, HRV3C

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Timmer JC, Salvesen GS. Methods Mol Biol. 2011;753:243-55.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200394	50319	1000 units	$357.50		Add to Cart

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