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Home / Proteins & Peptide

HMGB1, His, Human

SKU : BHP18500070

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iXCells Biotechnologies

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Mfr.No.	IX02803, IX02803-1, IX02803-50
Description	Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20 °C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Molecular Weight	Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 223 amino acids with 6 x His at C-terminus.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	Human
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Endotoxin Level	Less than 1 EU/ug of rHuHMGB1, His as determined by LAL method.

Synonyms	High-Mobility Group Box 1 (His6-tagged) ( HMGB1), Human;
Sequence	MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSEFSKKCSERWK?TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFKDPNAPKRPPS?AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADDKQPYEKKAAK?LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEEDEEDEEDEEEE?EDEEDEDEEE DDDDELEHHH HHH

Purity	95 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500070-1	IX02803-1	1mg	$1561		Add to Cart
BHP18500070-2	IX02803-50	50ug	$170		Add to Cart

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