Human HMGB1 also known as high-mobility group protein 1, and amphoterin, GenBank Accession No. NM_002128, a.a. 2-215, with C-terminal Avi-His-Tag, and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTagTM technology. MW = 25 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
2-215
Tag
C-terminal Avi-His-Tag, Biotin-labeled
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful as a positive control for Western blot studies.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_002128
Mw(kda)
25 kDa
Uniprot
P09429
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Purity
>=90%
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Scaffidi, P., et al., Nature. 2002; 418: 191-195.2. Lotze, M., et al., Nature. 2005; 5: 331-342.