5X mixture of full length human Histones H2A (GenBank Accession No. NM_033445), and H2B (NM_003528), each with N-terminal His-tag, expressed in E. coli. MW=14.8 kDa (H2A), 14.6 kDa (H2B).
Host cell line: E. coli
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
PBS, pH 7.4
Species
Human
Application
Optimized for use as a substrate for PARPs. Also useful as a substrate for histone acetyltransferases or methyltransferases.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_033445(H2A), NM_003528 (H2B)
Mw(kda)
14.8 kDa (H2A), 14.6 kDa (H2B)
Synonyms
Histone H2A, Histone H2B, PARP substrate
Uniprot
Q7L7L0 (H2A), Q16778 (H2B)
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Panzeter, P. L., et al. (1993). J. Biol. Chem. 268, 17662-17664. 2. Lee, J.S., et al. (2010). Cell 142(5):682-5. 3. Zhu, Q, Wani A.A. (2010). J. Cell Physiol. 223(2):283-8. 4. Campos, E.I., Reinberg, D. (2009). Annu. Rev. Genet. 43:559-99.