This peptide is histone H4 (1-23) monomethylated at Arg3 followed by a biotinylated Lys conjugated to a C-terminal GG linker. Methylation of Arg3 is catalyzed by PRMT1 and functions to promote p300 acetylation of histone H4.
Host cell line: synthetic
Immunogen Region
1-23
Tag
Biotin labeled
Species
Mammalian
Application
Useful as a substrate for arginine methyltransferases and for screening small molecular inhibitors of Histone 4 (R3) methyltransferases for drug discovery.
Concentration
200 uM
Notes
Avoid freeze/thaw cycles.
Amino Acid Sequence
SG-R(Me1)-GKGGKGLGKGGAKRHRKVLRGG-K(Biotin)
Mw(kda)
2843.4 Da
Uniprot
P62805
Shipping Temperature
gel packs (-20°C)
Purity
>=95% by HPLC
Storage
-20°C. Stable at least 1 year from date of receipt when stored as recommended.
Reference
Wang, H. et al. Science 293, 853 (2001).
Reviews of Histone H4 Peptide (1-23), Monomethyl R3, Biotin-labeled