Human recombinant histone H3 (a.a. 1-136(end)) with K27C, C97A, and C111A mutations, and an N-terminal His-tag, expressed in an E. coli expression system. GenBank Accession No. NM_003532. The protein has been alkylated to produce the lysine analog, methyl aminoethylcysteine. MW = 16 kDa.
Host cell line: E. coli
Immunogen Region
2-136(end)
Tag
N-terminal His-tag
Formulation
PBS (8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl), 20% glycerol.
Mutation
K27C
Species
Human
Application
A perfect substrate for histone methyltransferases and demethylases.