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Home / Proteins & Peptide

Histone H3/H4 Tetramer (full length), His-tag

SKU : BHP18201822

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BPS Bioscience

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Mfr.No.	52064-1
Description	Human recombinant histone tetramer, full length, consisting of 2 molecules each of histones H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), each with an N-terminal His-tag, expressed in an E. coli expression system. GenBank Accession Nos. are NM_003532, and NM_003548, respectively. Global MW = 57 kDa.
Background	The histone tetramer consists of two copies of both histones H3 and H4. It forms the central core of the nucleosome and, as such, plays a prominent role in the assembly, disassembly and positioning of nucleosomes.
Tag	N-terminal His-tag
Formulation	45 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA, 1 mM DTT and 10% glycerol.
Species	Human
Application	Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications.
Notes	Since the recombinant histone proteins in this tetramer were prepared in E. coli, they will not have methyl, acetyl or many other epigenetic marks. Avoid freeze/thaw cycles.
GenBank	NM_003532, NM_003548
Mw(kda)	57 kDa

Synonyms	H3/H4
Uniprot	P68431, P62805

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Huang, C., et al., PLoS Genet. 2013; 9(6): 1371. 2. Liu, W.H., et al., Nucleic Acids. 2012; 40(22):11229-39

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201822	52064-1	50 ug	$247.50		Add to Cart

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