Human Histone 3 (HIST1H3E), GenBank Accession No. NM_003532, a.a. 2-136(end) with N-terminal His-tag MW = 15.4 kDa, expressed in an E. coli expression system.
Host cell line: E. coli
Immunogen Region
2-136(end)
Tag
N-terminal His-tag
Formulation
8 mM PBS pH 7.4, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol
Species
Human
Application
Useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.
Notes
Histone H3.1 was coated onto a 96-well plate, and methylation by G9a was carried out at room temperature for 60 min. Methylated Histone H3.1 was detected using Chemiluminescence assay.
Avoid freeze/thaw cycles.
GenBank
NM_003532
Mw(kda)
15.4 kDa
Synonyms
Histone 3.1, HIST1H3E
Uniprot
P68431
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Loyola, A. et al., Mol. Cell 24 (2), 309-316 (2006). 2. Hans, F. and Dimitrov, S., Oncogene 20(24), 3021-3027 (2001).Product Specific Reference(s): 1. Morita, S., et al., Peptides. 48, 75-82 (2013).