Human HER4 kinase (ERBB4) (GenBank Accession No. NM_005235), a.a. 708-993, with N-terminal GST-tag, MW=59.5 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 48 pmol/min/ug.
Immunogen Region
708-993
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The enzyme reaction was carried out at 37°C for 20 minutes in a buffer containing 20 mM Tris-HCl, pH 7.4, 10 mM of MgCl2, 1 mM of MnCl2, 1 mM of DTT, 1 mM of ATP, and 1 ug/ml poly(Glu4-Tyr) peptide substrate.
Avoid freeze/thaw cycles.
GenBank
NM_005235
Mw(kda)
59.5 kDa
Synonyms
HER4, ERBB4, human epidermal growth factor receptor 4
Uniprot
Q15303
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Strunk, K.E. et al., Cancer Res. 67 (14), 6582- 6590 (2007). 2. Law, A.J. et al., Hum. Mol. Genet. 16 (2), 129-141 (2007).