Human Her2 (GenBank Accession No. X03363), a.a. 679-1255(end) with N-terminal GST-tag, MW=89.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 48 pmole/min/ug
Unit definition: One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Immunogen Region
679-1255
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed in Kinase buffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 uM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).